XYT Event Detector

XYT Event Detector detects and analyses time-dependent increases in intensity of a fluorescent indicator recorded by high-speed 2D cameras. Specifically, the program was developed and tuned to detect Local Calcium Releases in cardiac pacemaker cells. It works with stacks of tif images of Ca signals measured in living cells. The consecutive images in the stack represent the intensity of local Ca dynamics measured with a fixed sampling rate by a high-speed camera and a fluorescent Ca indicator. When pacemaker cells generate a cardiac impulse, the action potential induces a global Ca increase in cytoplasmic Ca (dubbed AP-induced Ca transient). The program identifies and eliminates global signal shifts and transients and detects only local signals. Read more about the XYT Event Detector .

Intramural Research Program XYT Event Detector Biophysics Biophysics Resources